Cl4-tcr
WebTo follow autoreactive T cells during AIH, we adoptively transferred HA--specific Cl4-TCR and 6.5-TCR T cells into Ad-Cre infected -Rosa26-HA mice. Alternatively, Rosa26-HA mice have been crossed with TCR transgenic mice that were infected with Ad-Cre to break hepatic tolerance and induce the expression of the HA antigen as a hepatic self-antigen. WebDec 1, 2000 · However, it is interesting that CL4-TCR CD8 + cells stimulated with APC and peptide can also lose the CD8 molecule, especially in light of the fact that activation of CL4-TCR-expressing T cells by Ag-pulsed APC or by soluble monomeric MHC-peptide complexes was found to be CD8-dependent (30, 36). Because the decrease in the …
Cl4-tcr
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WebCL4-TCR transgenes by polymerase chain reaction (PCR) using specific primers. Mice were bred and maintained under specific pathogen-free conditions and used for experiments at 6 to 18 weeks of age. All animal experi-ments were performed in agreement with local regula-tions. WebSep 1, 2008 · Section snippets Mice. VILLIN-HA mice express the A/PR8/34 HA from influenza virus A under control of the enterocyte-specific VILLIN promoter as described …
WebSep 17, 2015 · This system uses naive CD8 + T cells from CL4 TCR-transgenic mice expressing a TCR that specifically recognizes an epitope (aa 512-520) from influenza HA presented by H-2K d. Non-GVHD and GVHD mice received CL4 peptide or were infected with a recombinant MCMV expressing the CL4 epitope (MCMV-HA) 1 day after the … WebTo follow autoreactive T cells during AIH, we adoptively transferred HA--specific Cl4-TCR and 6.5-TCR T cells into Ad-Cre infected -Rosa26-HA mice. Alternatively, Rosa26-HA …
WebJan 31, 2008 · (referred to as GFAP-HA hereafter). HA peptides are expressed by EGCs and recognized by CD8 T cells that express a T-cell receptor (TCR) specific for K d:HA 512–520 (CL4) or CD4 T cells that … WebDownload scientific diagram Tumor growth in the class I-restricted CL4 TCR transgenic mice is not due to tolerization of T cells. In A, 8 10 6 T cells from CL4 mice that …
WebJan 31, 2008 · (referred to as GFAP-HA hereafter). HA peptides are expressed by EGCs and recognized by CD8 T cells that express a T-cell receptor (TCR) specific for K d:HA …
Thus, F5 TCR Tg mice have aided in vaccine design against viruses that undergo frequent mutation. Clone-4 (CL4) TCR Tg mice express TCR specific for the HA peptide epitope IYSTVASSL of the PR8 strain of influenza virus . Morgan et al. used this TCR Tg mouse strain to investigate the onset of peripheral tolerance induction upon influenza virus ... how old is sunkyWebAug 1, 2006 · Section snippets Mice. VILLIN-HA mice as described 7 express the A/PR8/34 HA from influenza virus A under control of the enterocyte-specific villin promoter. CL4 … how old is sunny hostin husbandWebFigure Legend Snippet: (A) CFSE-labeled HA-specific CD8 + CD25 − T cells from CL4-TCR transgenic mice were adoptively transferred into VILLIN-HA and BALB/c recipient mice. At day 4 after adoptive transfer cells from the MLN of recipient mice were isolated and stained for the expression of CD8 and Foxp3. how old is sunny from omoriWebResults: Transgenic CD8+ T cells from VILLIN-HA x CL4-TCR transgenic mice preferentially migrated and expanded in mucosal lymphoid tissues. Although transgenic CD8+ T cells … how old is sunny and melonhttp://img1.bioon.com/news/showarticle.asp?newsid=112634 how old is sunny emmerichWebMar 6, 2012 · transgenic mice (GFAP-HA CL4-TCR), one expressing influenza virus hemagglutinin (HA) in astrocytes and EGCs, and the other with CD8 T cells sensitized against HA and thus expressing the specific receptor. The resulting progeny showed a selective apoptotic depletion of EGCs mediated by a mech-anism of intestinal … how old is sunny balwaniWeb005308 B10.Cg- H2 d Tg(TcraCl4,TcrbCl4)1Shrm/ShrmJ These transgenic mice express a T-cell receptor specific for an epitope of hemagglutinin that is restricted by MHC class I … how old is sunny sandler