WebWash the membrane with washing buffer 3 times (10 minutes each time). Place the membrane in secondary antibody diluted in 1% BSA in Tween PBS, pH 7.2, and incubate at room temperature for 1 hour with shaking. Wash the membrane with washing buffer 3 times (10 minutes each time). Chemiluminescence method. WebWestern blotting is a technique that uses specific antibodies to identify proteins that have been separated based on size by gel electrophoresis. The immunoassay uses a membrane made of nitrocellulose or PVDF …
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